The use of melting curves as a novel approach for validation of real-time PCR instruments
Validation of PCR thermal cycler performance is crucial in order to obtain reliable results.In this study, high resolu- tion melting curve (HRM) analysis is presented as a novel validation method for real-time PCR instruments.By ap- plying HRM analysis using a defined PCR amplicon and EvaGreen dye, information about the temperature accuracy and the